Effect of modulation of hydrogen sulfur and glutathione synthesis on oxidative and nitrosative metabolism under myocardial ischemia-reperfusion

Redox balance maintaining is a prerequisite for normal cardiomyocytes metabolism. Reperfusion injury of the heart characterized by an explosive increase in ROS generation, cell membranes damage, dysfunction proteins, development muscle contracture etc. It was shown that hydrogen sulfide (H2S) and precursor its synthesis amino acid L-cysteine provided cardioprotective effect against ischemiareperfusion, In addition, one acids form antioxidant glutathione (GSH). The aim our work to investigate GSH H2S modulation on oxidative nitrosative stress cardiac tissues under conditions ischemia-reperfusion. inhibitor propargylglycine (PAG), butionine sulfoxime (BSO) were administered intraperitoneally. Next, ischemia-reperfusion Langendorff isolated rat performed. rat's hearts, before after ischemia, we determined rate content POL products, activity NO synthesizing enzymes. Results study showed pretreatment with PAG + combination prevented function disturbances, formation, increased low molecular weight nitrosothiols content, preserved constitutive NOS inhibited inducible pre-ischemic period as well reperfusion. Pretreatment BSO group significantly reduced effectiveness abolished effect. Generation superoxide hydroxyl anions increased, NO-synthase 3.5-fold comparing pretreated group. formation NOS, thus providing stable production NO. completely decreasing bioavailability glutathione.